I can't imagine a successful clinical lab without FCS Express it is the "go-to" Clinical package."įlow Cytometry Discipline Director Laboratory Corporation of America Holdings De Novo Software has been able to provide us with the features that we need and their technical support is extraordinary. I need a user friendly package that will accommodate our current complex multicolor analysis as well as be able to expand for future growth, provide high quality reports for our clients, offer a virtual component for those customers that want to offer tech only flow or more closely interact with their patient results and the big challenge is that this has to happen in an extremely high volume reference laboratory. In my opinion there just isn't another software option for us. Note: What I don't personally think is useful are some of the features benchling brings, such as primer design and CRISPR gRNA designs imo you'd be much better off with directly using published tools such as primer3, instead of the rather intransparent benchling toolbox."My function here at Labcorp is to set up a work flow process for over 350 cases a day companywide so without a doubt I am choosing FCS Express to accomplish this. a re-sequencing of a particular plasmid stock for project A or B). This could also be applied to "attached" chromatogram files (e.g. having a "plasmid list" containing your plasmids: you could link to the plasmid, then make an edit (or "mutagenesis") and store it as a new file then if you realize your plasmid promoter sequence is slightly different, edit the parent plasmid, and have this edit go through the "inherited" files (with notice/selection boxes). What I was missing when I last used it is "inherited" files.
sequences, alignments and chromatograms are individual files), and hierarchical folders allow straightforward separation of data between projects and analyses. In addition, the file/folder structure is intuitive (i.e. This, together with a great annotations tools including feature libraries, and a "copy annotations if identity threshold of >X%", makes sequence analysis, plasmid assembly and cloning workflows so much better.
Especially the alignment view, which can align chromatograms (ab1 files) to references, and these alignments/chromatogram basecalls can be easily edited/fixed. The hands-down best DNA sequence analysis tool.
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